Trout-liver histones
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چکیده
منابع مشابه
Trout-liver histones.
1. The histones of trout liver were examined and four main fractions (f1, f2b, f2a and f3) were isolated and characterized. 2. The amino acid analyses, N-terminal group analyses and starch-gel electrophoresis patterns are remarkably similar to the corresponding fractions of calf thymus. 3. The group f2a was also separated into two subfractions, f2al and f2a2, which are similar to those of calf ...
متن کاملModification of Histones during Spermatogenesis in Trout
Trout testis cells were incubated with 13H]arginine and lysine and inorganic [32P]phosphate. Histones were extracted with acid and the major fractions (I, IIbl, IIb2, III monomer, and IV) were resolved by chromatography on BioGel P-10. The profile of 32P incorporation was time dependent. When the phosphorylated histones in each of the major fractions were resolved from unphosphorylated species ...
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Acetylation of histones has been observed in both the spermatocytes and spermatids from developing trout testis. In the former cell type, both histone synthesis and extensive acetylation occur, whereas in the spermatids there is histone acetylation, but very little synthesis. The half-life of the histone acetyl groups is approximately 23 hours in the prespermatid cells. It is suggested that the...
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Turnover of whole histones of brain and liver nuclei was studied after administration of 14C-lysine to adult mice. The radioactivity of histones and other nuclear and cytoplasmic proteins was determined at intervals from 2 hours to 245 days. Two fractions of cerebral histones with halflifetimes of 54 and 104 days and three fractions of hepatic histones with half-lifetimes of 18, 56, and 93 days...
متن کاملIsocitrate Dehydrogenases in Rainbow-trout Liver
1. The kinetics of the thermally induced enzyme variants of the supernatant NADP-isocitrate dehydrogenase from rainbow-trout liver are investigated. 2. Fish acclimatized to 20C (cold-adapted enzyme) and 170C (warm-adapted enzyme) show different relative distributions of the three NADP-isocitrate dehydrogenase isoenzymes; this has been demonstrated with electrophoresis and electrofocusing techni...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1966
ISSN: 0006-2936
DOI: 10.1042/bj1000779